In the early 1950s, experiments by two teams of researchers, Salvador Luria working with Mary Human and Joe Bertani working with Jean Weigle, showed that some strains of bacteria were more resistant ...
A Nobel laureate, he identified an enzyme that cuts DNA, laying the groundwork for milestones in scientific research and ...
The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...
Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...
Unlike other restriction enzymes, BfiI functions without metal ions. It recognizes an asymmetric DNA sequence, 5′-ACTGGG-3′, and cuts top and bottom strands at fixed positions downstream of this ...
Grapevine dieback, caused by Lasiodiplodia theobromae, is an important trunk disease worldwide. Transformants of L. theobromae were generated in an attempt to identify potential pathogenicity-related ...
TALENs involve the synthesis of the C-terminal with type II restriction enzyme, Fokl, in order to produce heterodimers that form a double-stranded break (DSB) in DNA. Recent advancements in TALEN ...
Geoff Wilson, Senior Scientist and Division Head of the Restriction Enzyme Division at New England Biolabs, describes these fascinating enzymes from the perspective of someone who has been there from ...
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